ACID MUCOPOLYSACCHARIDES AS DISPERSING AGENTS IN LYSOSOMAL DIGESTION

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Acid mucopolysaccharides as cofactor in formation of platelet-clumping substance.

Incubation at 37#{176}Cfor 30 mm or the addition of CaCl2 (5 mM) generated platelet-clumping activity in citrated plasma and EDTA plasma previously acidified to pH 5.5 and in which heparin was added to give a concentration of 0.5 mg/mi. Similar treatment could not produce platelet-clumping activity in saline or denatured plasma that had been heated to 56#{176}C.Heparin, chondroitin sulfate B, o...

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Acid Mucopolysaccharides As Cofactor in Formation of Platelet - clumping Substance

Incubation at 37#{176}Cfor 30 mm or the addition of CaCl2 (5 mM) generated platelet-clumping activity in citrated plasma and EDTA plasma previously acidified to pH 5.5 and in which heparin was added to give a concentration of 0.5 mg/mi. Similar treatment could not produce platelet-clumping activity in saline or denatured plasma that had been heated to 56#{176}C.Heparin, chondroitin sulfate B, o...

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Acid mucopolysaccharides in normal serum.

Mucopolysaccharide which contains uronic acid has recently been described in normal plasma (1, 2). A method for the quantitation of plasma acid mucopolysaccharide was described by Badin, Schubert and Vouras (1), based on the observation that chondroitin sulfate added to plasma is found in euglobulin precipitates (3). These authors reported an increased amount of acid mucopolysaccharide in the p...

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Quantitative Colorimetric Assay of Acid Mucopolysaccharides

In 1946 Hale (1) described a histochemical method for the detection of acid polysaccharides in animal tissues, based on the ability of these compounds to bind ferric ions, which were subsequently detected as Prussian blue. It has been possible to adapt this histochemical method to the quantitative determination of acid mucopolysaccharides on a macro or micro scale. The major modification of the...

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Quantitative Colorimetric Assay of Acid Mucopolysaccharides

In 1946 Hale (1) described a histochemical method for the detection of acid polysaccharides in animal tissues, based on the ability of these compounds to bind ferric ions, which were subsequently detected as Prussian blue. It has been possible to adapt this histochemical method to the quantitative determination of acid mucopolysaccharides on a macro or micro scale. The major modification of the...

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ژورنال

عنوان ژورنال: Pediatric Research

سال: 1974

ISSN: 0031-3998,1530-0447

DOI: 10.1203/00006450-197404000-00128